Isolation of a distally located gene possibly correlated with gametocyte production ability
Mem. Inst. Oswaldo Cruz
; 89(Suppl.2): 33-35, 1994.
Article
em En
| LILACS
| ID: lil-319955
Biblioteca responsável:
BR1.1
RESUMO
Previous studies were focussed on the attempt to correlate observable variations in the size of Plasmodium berghei chromosomes with the loss of ability to produce viable gametocytes. A temporal coincidence between the appearance of a subtelomeric deletion on P. berghei chromosome 5 and the loss of the ability to produce viable gametocytes was observed in a clone (HPE) directly derived from the high gametocyte-producer clone 8417 during mechanical passages. Interestingly enough, three P. berghei sexual-specific genes have already been mapped on internal fragments of this chromosome. A novel gene, clone 150, isolated from a genomic library of clone 8417 using a probe enriched for sexual-specific transcripts, maps on chromosome 5 within 100kb from the telomere. Subtelomeric deletions of chromosome 5 affecting two non-producer clones involve part of the transcribed region of this gene.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Plasmodium berghei
/
Mapeamento Cromossômico
/
Genes de Protozoários
/
Gametogênese
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
1994
Tipo de documento:
Article