Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale
Mem. Inst. Oswaldo Cruz
; 100(7): 765-769, Nov. 2005. ilus, tab
Article
em En
| LILACS
| ID: lil-419704
Biblioteca responsável:
BR1.1
ABSTRACT
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant MSP1a and MSP2 from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. The high sensitivities (99 percent for both tests) and specificities (100 percent for both tests) were confirmed with sera from cattle positive or negative for A. marginale antibodies, respectively, by immunofluorescent antibody test. By the analysis of 583 sera from cattle of three regions of the state of Pernambuco, Brazil, the agreement between both tests was high, with a kappa index of 0.89. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Ensaio de Imunoadsorção Enzimática
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Doenças dos Bovinos
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Anaplasma marginale
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Anaplasmose
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Anticorpos Antibacterianos
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Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2005
Tipo de documento:
Article