Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A. m. lamarckii
Genet. mol. biol
; 29(4): 747-749, 2006. tab
Article
em En
| LILACS
| ID: lil-450500
Biblioteca responsável:
BR26.1
ABSTRACT
Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial "O" lineage, rather than a diagnostic site for A. m. lamarckii.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Abelhas
/
Complexo IV da Cadeia de Transporte de Elétrons
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Genet. mol. biol
Ano de publicação:
2006
Tipo de documento:
Article
/
Project document