Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene

Carnieli Junior, P; Ventura, A. M; Durigon, E. L

Carnieli Junior, P; Ventura, A. M; Durigon, E. L.

Afiliação

Carnieli Junior, P; Pasteur Institute. São Paulo. BR
Ventura, A. M; University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology. São Paulo. BR
Durigon, E. L; University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology. São Paulo. BR

J. venom. anim. toxins incl. trop. dis ; 13(4): 874-880, 2007. ilus

Article em En | LILACS | ID: lil-471147

Biblioteca responsável: BR33.1

ABSTRACT

ABSTRACT

A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.

Assuntos

Glicoproteínas/genética; Plasmídeos; Proteínas Recombinantes de Fusão/genética; Vírus da Raiva; Transfecção

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: LILACS Assunto principal: Plasmídeos / Vírus da Raiva / Proteínas Recombinantes de Fusão / Glicoproteínas / Transfecção Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Ano de publicação: 2007 Tipo de documento: Article

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