Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Mem. Inst. Oswaldo Cruz
; 104(1): 118-120, Feb. 2009. ilus
Artigo
em Inglês
| LILACS
| ID: lil-507216
Biblioteca responsável:
BR1.1
ABSTRACT
Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Infecções Respiratórias
/
Adenoviridae
/
Nasofaringe
/
Vírus Sincicial Respiratório Humano
/
Infecções por Vírus Respiratório Sincicial
/
Infecções por Adenoviridae
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
/
Estudo prognóstico
Limite:
Criança
/
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2009
Tipo de documento:
Artigo
/
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