Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1
Braz. j. microbiol
; 43(1): 177-186, Jan.-Mar. 2012. ilus, tab
Article
em En
| LILACS
| ID: lil-622802
Biblioteca responsável:
BR32.1
ABSTRACT
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Carbono
/
Quitinases
/
Interpretação Estatística de Dados
/
Fermentação
/
Inulina
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Braz. j. microbiol
Ano de publicação:
2012
Tipo de documento:
Article