A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
Braz. j. infect. dis
; Braz. j. infect. dis;17(6): 667-671, Nov.-Dec. 2013. ilus, tab
Article
em En
| LILACS
| ID: lil-696968
Biblioteca responsável:
BR1.1
ABSTRACT
OBJECTIVE:
To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis.METHODS:
Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction.RESULTS:
DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing.CONCLUSIONS:
The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
LILACS
Assunto principal:
Tuberculose Resistente a Múltiplos Medicamentos
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Reação em Cadeia da Polimerase Multiplex
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Mycobacterium tuberculosis
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Antituberculosos
Tipo de estudo:
Diagnostic_studies
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Health_economic_evaluation
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Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Braz J Infect Dis
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Braz. j. infect. dis
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Braz. j. infect. dis. (Online)
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Brazilian journal of infectious diseases (Impresso)
Ano de publicação:
2013
Tipo de documento:
Article