Differential expression of immunologic proteins in gingiva after socket preservation in mini pigs
J. appl. oral sci
; 23(2): 187-195, Mar-Apr/2015. graf
Article
em En
| LILACS, BBO
| ID: lil-746533
Biblioteca responsável:
BR1.1
ABSTRACT
During healing following tooth extraction, inflammation and the immune response within the extraction socket are related to bone resorption. Objective:
We sought to identify how the alloplastic material used for socket preservation affects the immune responses and osteoclastic activity within extraction sockets. Material andMethods:
Using a porcine model, we extracted teeth and grafted biphasic calcium phosphate into the extraction sockets. We then performed a peptide analysis with samples of gingival tissue from adjacent to the sockets and compared the extraction only (EO) and extraction with socket preservation (SP) groups. We also used real-time polymerase chain reaction (PCR) to evaluate the expression level of immunoglobulins, chemokines and other factors related to osteoclastogenesis. Differences between the groups were analyzed for statistical significance using paired t tests.Results:
Levels of IgM, IgG and IGL expression were higher in the EO group than in the SP group 1 week post-extraction, as were the levels of CCL3, CCL5, CXCL2, IFN-γ and TNF-α expression (p<0.05). In addition, receptor activator of nuclear factor kappa-B ligand (RANKL) was also significantly upregulated in the EO group (p<0.05), as were IL-1β, IL-6 and IL-8 (p<0.05).Conclusions:
These results suggest that the beneficial effect of socket preservation can be explained by suppression of immune responses and inflammation. .Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
BBO
/
LILACS
Assunto principal:
Encéfalo
/
Imageamento por Ressonância Magnética
Tipo de estudo:
Prognostic_studies
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J. appl. oral sci
Ano de publicação:
2015
Tipo de documento:
Article