Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
Mem. Inst. Oswaldo Cruz
; 110(4): 577-579, 09/06/2015. graf
Artigo
em Inglês
| LILACS
| ID: lil-748866
Biblioteca responsável:
BR1.1
ABSTRACT
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Imunoglobulinas
/
Vírus da Hepatite A
/
Hepatite A
/
Fígado
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Aspecto:
Aspectos éticos
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Ano de publicação:
2015
Tipo de documento:
Artigo
/
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