Voltammetric measurement of blood nitric oxide in irradiated rats.
Int J Radiat Biol
; 75(2): 201-8, 1999 Feb.
Article
em En
| MEDLINE
| ID: mdl-10072181
ABSTRACT
UNLABELLED PURPOSE. To investigate the effect of blood nitric oxide (NO) as a mediator of the neurovascular syndrome in rats following gamma-irradiation. MATERIAL AND METHODS:
Using a voltametric method together with a carbon fibre based sensor, NO measurements were carried out in sham-irradiated and irradiated animals either in blood from the abdominal aorta or in blood samples from the heart.RESULTS:
In in vitro conditions, properties of the probe were not altered by the ionizing radiation. Significant increases of +17% and +25.6% were observed in the voltametric signal height at 90 min and 24 h respectively after a 15 Gy gamma-ray exposure. These effects were followed on days 3 and 4 by a progressive decrease in the signal height of 7% and 18% respectively. Dose-effect relationships were observed at 90 min and 24 h after exposure to gamma-rays in the range of 3-15 Gy. Finally, the NO dependence on the measured voltametric signal was controlled by using inhibitors of the NO synthase (NOS) and by performing nitrate assays.CONCLUSIONS:
Specific blood NO voltametric measurements are possible. Functional changes associated with NO after gamma-ray exposure are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Irradiação Corporal Total
/
Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
Int J Radiat Biol
Ano de publicação:
1999
Tipo de documento:
Article