Induction of a protective immunity against Schistosoma mansoni with ovalbumin-coupled Sm37-5 coadsorbed with granulocyte-macrophage colony stimulating factor (GM-CSF) or IL-12 on alum.
Vaccine
; 17(1): 13-8, 1999 Jan.
Article
em En
| MEDLINE
| ID: mdl-10078602
ABSTRACT
A previous study has shown that Sm37-5 is a major B cell epitope of Sm37-GAPDH. This epitope is highly antigenic in human infections and IgG antibody reactivity toward this determinant is associated with adolescent resistance to reinfection. This led us to test a synthetic peptide corresponding to Sm37-5, coupled to ovalbumin, as an anti-schistosome vaccine. Although mice injected with Sm37-5-OVA in Freund's adjuvant showed significant protection, immunization in aluminium hydroxide failed to induce protection. The adjuvant effect of cytokines (GM-CSF or IL-12) associated with the antigen on alum was investigated. With each of these two cytokines, significant reductions in the worm burden were obtained (32-38% with GM-CSF and 27% with IL-12, respectively). In addition, a reduction of the egg number trapped in the liver of immunized mice was also observed. Thus, protections were obtained with formulations that could potentially be used in humans.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Schistosoma mansoni
/
Esquistossomose mansoni
/
Ovalbumina
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
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Interleucina-12
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Epitopos de Linfócito B
/
Compostos de Alúmen
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
1999
Tipo de documento:
Article