Prox1 function is crucial for mouse lens-fibre elongation.
Nat Genet
; 21(3): 318-22, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10080188
ABSTRACT
Although insights have emerged regarding genes controlling the early stages of eye formation, little is known about lens-fibre differentiation and elongation. The expression pattern of the Prox1 homeobox gene suggests it has a role in a variety of embryonic tissues, including lens. To analyse the requirement for Prox1 during mammalian development, we inactivated the locus in mice. Homozygous Prox1-null mice die at mid-gestation from multiple developmental defects; here we describe the specific effect on lens development. Prox1 inactivation causes abnormal cellular proliferation, downregulated expression of the cell-cycle inhibitors Cdkn1b (also known as p27KIP1) and Cdkn1c (also known as p57KIP2), misexpression of E-cadherin and inappropriate apoptosis. Consequently, mutant lens cells fail to polarize and elongate properly, resulting in a hollow lens. Our data provide evidence that the progression of terminal fibre differentiation and elongation is dependent on Prox1 activity during lens development.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Homeodomínio
/
Regulação da Expressão Gênica no Desenvolvimento
/
Proteínas de Ciclo Celular
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas Supressoras de Tumor
/
Cristalino
Limite:
Animals
Idioma:
En
Revista:
Nat Genet
Ano de publicação:
1999
Tipo de documento:
Article