Whole-column radioactivity detection: simultaneous separation and enhanced detectability.
Anal Chem
; 71(14): 2700-7, 1999 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-10424163
ABSTRACT
The development of a whole-column radiation detector for measurement and separation of low amounts of beta emitting analytes is described. The design of this detector is unique with all of the chromatography media located within the detector volume. This whole-column design provides the advantage of increased radiation signal without loss of chromatographic efficiency, which translates to increased detectability. This increase was compared theoretically with flow-through radiation detection, and the theory was tested experimentally. Using two analytes, carbon-11-labeled m-hydroxyephedrine and alpha-methylepinephrine, only 3 and 8 Bq (80 and 220 pCi), respectively, were needed to obtain a 10% coefficient of variation using whole-column detection. For [11C]-m-hydroxyephedrine, 100 times more radioactivity was required to achieve the same coefficient of variation using flow-through detection. A limit of detection (LD) for the analytes of 2 Bq (54 pCi) was obtained for whole-column detection, an improvement of 50 in LD compared with flow-through detection. Signal improvement increased linearly with the chromatographic resolution. The whole-column detection method is robust and applicable to many chromatographic separations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radiometria
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
1999
Tipo de documento:
Article