Impaired growth on glucose of a pyruvate dehydrogenase-negative mutant of Kluyveromyces lactis is due to a limitation in mitochondrial acetyl-coenzyme A uptake.
FEMS Microbiol Lett
; 177(1): 23-8, 1999 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-10436919
ABSTRACT
A Kluyveromyces lactis mutant with a disruption in the KlPDA1 gene, encoding the E1 alpha subunit of the pyruvate dehydrogenase complex, exhibited a four-fold reduced specific growth rate on glucose in minimal medium. Growth of the Klpda1 mutant on glucose in complex medium was not affected. Its growth on defined media could be restored by adding amino acids that require mitochondrial acetyl-CoA for their biosynthesis as nitrogen sources. This, together with the observation that low-concentrations of L-carnitine also restored growth on glucose, indicates that the slow-growth phenotype of the Klpda1 mutant is due to a limited capacity of the mitochondria for import of cytosolic acetyl-CoA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilcoenzima A
/
Complexo Piruvato Desidrogenase
/
Kluyveromyces
/
Glucose
/
Mitocôndrias
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1999
Tipo de documento:
Article