Targeting an adenoviral gene vector to cytokine-activated vascular endothelium via E-selectin.
Gene Ther
; 6(5): 801-7, 1999 May.
Article
em En
| MEDLINE
| ID: mdl-10505104
ABSTRACT
We have aimed at selective gene delivery to vascular endothelial cells (EC) at sites of inflammation, by targeting E-selectin, a surface adhesion molecule that is only expressed by activated EC. An anti-E-selectin mAb, 1.2B6, was complexed with the adenovirus vector AdZ.FLAG (expressing the FLAG peptide) by conjugating it to an anti-FLAG mAb. Gene transduction of cultured EC was increased 20-fold compared with AdZ.FLAG complexed with a control bsAb providing EC were activated by cytokines. The anti-E-selectin-complexed vector transduced 29 +/- 9% of intimal EC in segments of pig aorta cultured with cytokines ex vivo, compared with less than 0.1% transduced with the control construct (P < 0.05). This strategy could be developed to target endothelium in inflammation with genes capable of modifying the inflammatory response.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasculite
/
Endotélio Vascular
/
Terapia Genética
/
Adenoviridae
/
Selectina E
/
Vetores Genéticos
Limite:
Animals
Idioma:
En
Revista:
Gene Ther
Ano de publicação:
1999
Tipo de documento:
Article