Nitric oxide synthase-II is expressed in severe corneal alkali burns and inhibits neovascularization.
Invest Ophthalmol Vis Sci
; 40(12): 2773-9, 1999 Nov.
Article
em En
| MEDLINE
| ID: mdl-10549635
ABSTRACT
PURPOSE:
Inducible nitric oxide synthase (NOS-II) is expressed in many inflammatory conditions. The implication of nitric oxide (NO) in angiogenesis remains controversial. The role of NOS-II and its influence on angiogenesis in corneal neovascularization is unknown and was investigated in this study.METHODS:
A mouse model of corneal neovascularization induced by chemical cauterization was used. NOS-II mRNA expression was analyzed by reverse transcriptase-polymerase chain reaction, and NOS-II protein was studied in situ by immunohistochemical analysis of the cornea. The influence of NOS-II on neovascularization was determined by comparison of vessel development in "normal" wild-type mice and mice with a targeted disruption of the NOS-II gene.RESULTS:
NOS-II mRNA was induced to very high levels after corneal cauterization and remained upregulated throughout the disease. Migratory cells in the center of the cauterization area expressed NOS-II protein. The neovascular response in mice lacking the NOS-II gene was significantly stronger than in wild-type mice, and the difference increased over time.CONCLUSIONS:
These data are the first evidence that NOS-II is expressed in this model of sterile corneal inflammation. NOS-II expression inhibited angiogenesis in severe corneal alkali burns.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Queimaduras Químicas
/
Queimaduras Oculares
/
Neovascularização da Córnea
/
Óxido Nítrico Sintase
/
Córnea
Limite:
Animals
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Ano de publicação:
1999
Tipo de documento:
Article