Characterization of a chromosomally complex lung cancer cell line using multiwell fluorescence in situ hybridization.
Cancer Genet Cytogenet
; 117(2): 149-52, 2000 Mar.
Article
em En
| MEDLINE
| ID: mdl-10704688
ABSTRACT
The chromosomal characterization of a non-small cell lung cancer cell line (NCIH358) is described. This characterization was achieved using a simple, cheap and technically straightforward multiwell fluorescence in situ hybridization (FISH) method. The many and complex chromosome rearrangements identified by this method could not be defined using conventional G-banded chromosome analysis, and have not been previously described. For the detailed characterization of complex cell lines, multiwell FISH has many advantages over more technically demanding and expensive FISH techniques, and opens up the possibility of screening for consistent rearrangements, leading to the identification of unique fusion genes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aberrações Cromossômicas
/
Hibridização In Situ
/
Carcinoma Pulmonar de Células não Pequenas
/
Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Revista:
Cancer Genet Cytogenet
Ano de publicação:
2000
Tipo de documento:
Article