Characterization and functional role of leptin receptor in bovine adrenal medullary cells.
Biochem Pharmacol
; 59(9): 1141-5, 2000 May 01.
Article
em En
| MEDLINE
| ID: mdl-10704944
ABSTRACT
We report here the characterization and functional roles of the leptin receptor (ObR) in bovine adrenal medullary cells. The plasma membranes isolated from bovine adrenal medulla showed a single class of specific binding sites of (125)I-leptin with an apparent K(d) of 6.6 nM and B(max) of 62 fmol/mg protein. ObRa but not ObRb mRNA was detected in bovine adrenal medulla by reverse transcriptase-polymerase chain reaction. Incubation of cultured adrenal medullary cells with leptin (3-30 nM) for 20 min resulted in a significant increase in [(14)C]catecholamine synthesis from [(14)C]tyrosine without any change in catecholamine secretion. These findings suggest that leptin stimulates catecholamine synthesis through its receptors in bovine adrenal medullary cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Medula Suprarrenal
/
Receptores de Superfície Celular
/
Leptina
Limite:
Animals
Idioma:
En
Revista:
Biochem Pharmacol
Ano de publicação:
2000
Tipo de documento:
Article