Melanogenesis from 5-hydroxytryptamine, 5,6- and 5,7-dihydroxytryptamines. An in vitro study using MALDI-TOF.
Adv Exp Med Biol
; 467: 779-87, 1999.
Article
em En
| MEDLINE
| ID: mdl-10721131
ABSTRACT
The role of tyrosinase and peroxidase in melanogenesis of 5-hydroxytryptamine, 5,6- and 5,7-dihydroxytryptamines was investigated by matrix-assisted laser desorption/ionization mass spectrometry. Each enzyme was incubated with the tryptamine derivatives and samples were drawn at various times, ultrafiltered and immediately lyophilized. The results indicated that peroxidase promotes oligomerization of 5-HT with fast kinetics but with yields lower than those achieved by tyrosinase. 5,6- and 5,7-DHT formed low molecular mass oligomers in the presence of peroxidase alone. The addition of hydrogen peroxide evidences different reactivity of the two isomers 5,6-DHT formed immediately a black precipitate while oligomers of the molecule itself and of its oxidation products were detectable for 5,7-DHT.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Serotonina
/
5,6-Di-Hidroxitriptamina
/
5,7-Di-Hidroxitriptamina
/
Melaninas
Idioma:
En
Revista:
Adv Exp Med Biol
Ano de publicação:
1999
Tipo de documento:
Article