Solid-phase microextraction in the determination of methadone in human saliva by gas chromatography-mass spectrometry.
J Anal Toxicol
; 24(2): 93-6, 2000 Mar.
Article
em En
| MEDLINE
| ID: mdl-10732946
ABSTRACT
Solid-phase microextraction (SPME) with a 100-microm polydimethylsiloxane film fiber was applied to the determination of methadone and 2-ethylidine-3,3-diphenylpyrrolidine (EDDP) by GC-MS in human saliva and compared with liquid-liquid extraction. A shorter extraction time of 30 min with the fiber was obtained, speeding up the total analysis time. Linearity was found for SPME from 0.05 to 2.0 microg/mL (r = 0.9976 for methadone; r = 0.9988 for EDDP) with precision between 0.7 and 4.3% for saliva spiked with 0.2 and 1.5 microg/mL of methadone and EDDP. The limit of detection using SPME was 0.04 microg/mL for methadone and 0.008 microg/mL for EDDP. Analytical recoveries of SPME and liquid-liquid extraction ranged from 98.8 to 103.6%. The use of deuterated internal standard by both methods have yielded comparable results. Thus, the SPME method is highly accurate, precise, and useful for determination of methadone and EDDP in saliva.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Cromatografia Gasosa-Espectrometria de Massas
/
Metadona
Limite:
Humans
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
2000
Tipo de documento:
Article