An in vitro model of ischemic-like stress in retinal pigmented epithelium cells: protective effects of antioxidants.
Mech Ageing Dev
; 114(3): 185-90, 2000 Apr 14.
Article
em En
| MEDLINE
| ID: mdl-10802122
ABSTRACT
We have developed a model of in vitro cell oxidative stress in bovine retinal pigment epithelium cells exposed to a ischemia-like condition obtained by interference with glucose utilization through both oxidative phosphorylation and glycolysis. This resulted in a statistically significant decrease of the intracellular ATP levels, which reflects a bioenergetic decline similar to that associated with mitochondrial damage or loss in normal post-mitotic cells aging in vivo. This new model of cellular oxygen stress seems adequate for investigation of the protective action of antioxidants, in agreement with our finding of a statistically significant increase in the ATP levels over the values of the non-treated samples in retinal pigment epithelium cells exposed to the above oxygen stress in medium supplemented with 300 microM vitamin C or 10 mM N-acetylcysteine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Epitélio Pigmentado Ocular
/
Vasos Retinianos
/
Estresse Oxidativo
/
Isquemia
/
Antioxidantes
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mech Ageing Dev
Ano de publicação:
2000
Tipo de documento:
Article