Macrocyclic chelators with paramagnetic cations are internalized into mammalian cells via a HIV-tat derived membrane translocation peptide.
Bioconjug Chem
; 11(3): 301-5, 2000.
Article
em En
| MEDLINE
| ID: mdl-10821645
ABSTRACT
A major obstacle to using paramagnetic MR contrast agents for in vivo cell tracking or molecular sensing is their generally low cellular uptake. In this study, we show that a paramagnetically labeled DOTA chelator derivatized with a 13-mer HIV-tat peptide is efficiently internalized into mammalian cells. Intracellular concentrations were attained that were readily detectable by MR imaging using both gadolinium and dysprosium chelates. Using this paradigm, it should be feasible to internalize a variety of chemically different agents into mammalian cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Imageamento por Ressonância Magnética
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Produtos do Gene tat
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Quelantes
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Compostos Heterocíclicos com 1 Anel
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Bioconjug Chem
Ano de publicação:
2000
Tipo de documento:
Article