Interacting effects of capsaicin and anandamide on intracellular calcium in sensory neurones.
Neuroreport
; 11(9): 1949-52, 2000 Jun 26.
Article
em En
| MEDLINE
| ID: mdl-10884049
ABSTRACT
Capsaicin (100 nM to 1 microM) and anandamide (200 nM to 10 microM) caused a transient increase in fluorescence of fura-2 loaded cultured small trigeminal neurones of rats measured with a ratiometric technique. The percentage of cells responding to capsaicin at 100 nM, 330 nM and 1 microM was 47.4%, 45.3%, and 70.4%, respectively. Averaged peak value of fluorescense ratio (R) at 340 and 380 nm excitation was slightly dose dependent. Peaks of anandamide-induced transients were R = 0.2 at 200 nM and 0.16 at 10 microM. Near 40% of capsaicin-sensitive cells responded also to anandamide. Anandamide (200 nM) inhibited the capsaicin-induced calcium influx. The results suggest that anandamide increases intracellular calcium and inhibits capsaicin-evoked calcium transients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bloqueadores dos Canais de Cálcio
/
Capsaicina
/
Ácidos Araquidônicos
/
Cálcio
/
Membranas Intracelulares
/
Neurônios Aferentes
Limite:
Animals
Idioma:
En
Revista:
Neuroreport
Ano de publicação:
2000
Tipo de documento:
Article