Parallel induction of epithelial surface-associated chemokine and proteoglycan by cellular hypoxia: implications for neutrophil activation.
J Leukoc Biol
; 68(2): 251-9, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-10947070
ABSTRACT
Neutrophil-induced damage to the protective epithelium has been implicated in mucosal disorders associated with hypoxia, and such damage may be initiated by epithelial-derived chemokines. Because chemokines can bind to membrane proteoglycans, we hypothesized that chemokines may associate with epithelial surfaces and activate polymorphonuclear neutrophils (PMN). Epithelial hypoxia (pO2 20 torr) resulted in a time-dependent induction of interleukin-8 (IL-8) mRNA, soluble protein, as well as surface protein. Such surface IL-8 expression was demonstrated to be dependent on heparinase III expression, and extensions of these experiments indicated that hypoxia induces epithelial perlecan expression in parallel with IL-8. Finally, co-incubation of post-hypoxic epithelia with human PMN induced IL-8-dependent expression of the PMN beta2-integrin CD11b/18. These data indicate that chemokines liberated from epithelia may exist in a surface-bound, bioactive form and that hypoxia may regulate proteoglycan expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeo-Liases
/
Interleucina-8
/
Ativação de Neutrófilo
/
Proteoglicanas de Heparan Sulfato
/
Mucosa Intestinal
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
2000
Tipo de documento:
Article