Bactericidal action of high-power Nd:YAG laser light on Escherichia coli in saline suspension.
J Appl Microbiol
; 89(3): 517-25, 2000 Sep.
Article
em En
| MEDLINE
| ID: mdl-11021585
ABSTRACT
Infra-red light (1064 nm) from a high-power NdYAG laser caused more than 90% loss of viability of Escherichia coli during exposures that raised the temperature of PBS suspensions of the bacteria to 50 C in a thermocouple-equipped cuvette. In contrast, there was minimal loss of viability after heating the same suspensions to 50 degrees C in a water-bath, or in a PCR thermal cycler. The mechanism of laser killing at 50 degrees C was explored by differential scanning calorimetry, by laser treatment of transparent and turbid bacterial suspensions, and by optical absorbency studies of E. coli suspensions at 1064 nm. Taken together, the data suggested that the bactericidal action of NdYAG laser light at 50 degrees C was due partly to thermal heating and partly to an additional, as yet undefined, mechanism. Scanning electron microscopy revealed localized areas of surface damage on laser-exposed E. coli cells.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli
/
Lasers
Idioma:
En
Revista:
J Appl Microbiol
Ano de publicação:
2000
Tipo de documento:
Article