Determination of sugar alcohols in confectioneries by high-performance liquid chromatography after nitrobenzoylation.
J Chromatogr A
; 893(1): 195-200, 2000 Sep 29.
Article
em En
| MEDLINE
| ID: mdl-11043600
ABSTRACT
A method was developed for the determination of sugar alcohols, meso-erythritol, xylitol, D-glucitol, D-mannitol, maltitol and parachinit by high-performance liquid chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride. HPLC was performed on a phenyl column, using acetonitrile-water (6733) as mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10-250 microg/ml range. The average recoveries of the sugar alcohols from four sugarless confectioneries spiked at 5 and 10% levels of six sugar alcohol standards ranged from 73.2 to 109.0% with relative standard deviations ranging from 0.7 to 9.0%. The detection limit of the developed method was 0.1% for the above sugar alcohols contained in the samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Álcoois Açúcares
/
Cromatografia Líquida de Alta Pressão
/
Análise de Alimentos
/
Nitrobenzoatos
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2000
Tipo de documento:
Article