[Construction of a transformation-competent artificial chromosome (TAC) library of a wheat-Haynaldia villosa translocation line].
Sheng Wu Gong Cheng Xue Bao
; 16(4): 433-6, 2000 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-11051813
ABSTRACT
Transformation-competent artificial chromosome (TAC) vector is able to clone and transfer large DNA fragments in plants and is a powerful tool for plant gene isolation and transformation. To clone important genes from wheat, a TAC genomic library was constructed from nuclear DNA of a 6VS/6AL wheat-Haynaldia villosa translocation line that harbor the gene Pm21 for resistance to powdery mildew. The library consists of 2.1 x 10(6) clones with an average DNA insert size of 35 kb, and represents in total 4.9 genome equivalents. The library was stored as clone pools in 96-well plates, and each pool contained about 1000 clones. TAC clones containing gene(s) of interest can be screened by a pooled-PCR/colony-hybridization strategy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transformação Genética
/
Translocação Genética
/
Triticum
/
Biblioteca Gênica
/
Cromossomos Artificiais
Idioma:
Zh
Revista:
Sheng Wu Gong Cheng Xue Bao
Ano de publicação:
2000
Tipo de documento:
Article