In situ zymography: topographical considerations.
J Biochem Biophys Methods
; 47(3): 169-76, 2001 Feb 26.
Article
em En
| MEDLINE
| ID: mdl-11245888
ABSTRACT
In situ gelatin zymography is a simple technique providing valuable information about the cellular and tissue localization of gelatinases. Until recently, the use of this technique has been confined to soft, relatively homogeneous tissue. In this report in situ zymography has been utilized to assess the sub-lamellar location of gelatinases in the hard, semi-keratinized epidermal layer and the adjacent soft connective tissue matrix of the dermis of the equine hoof. We show that alterations in the orientation at which the tissue is dipped and withdrawn from the emulsion cause profound alterations in emulsion thickness. Microscopic variations in the surface topography of frozen tissue sections also influence emulsion thickness making interpretation of the results difficult. Given these results, researchers must be aware of potential variations in zymographic analysis may be influenced by physical tissue parameters in addition to suspected gelatinase activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gelatinases
Limite:
Animals
Idioma:
En
Revista:
J Biochem Biophys Methods
Ano de publicação:
2001
Tipo de documento:
Article