Survival of tubercle bacilli in heat-fixed and stained sputum smears.
Mem Inst Oswaldo Cruz
; 96(2): 277-80, 2001 Feb.
Article
em En
| MEDLINE
| ID: mdl-11285510
ABSTRACT
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Escarro
/
Corantes
/
Temperatura Alta
/
Micobactérias não Tuberculosas
Limite:
Humans
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
2001
Tipo de documento:
Article