Effect of combined treatment with radiation and low dose etoposide on cell survival.
Anticancer Res
; 21(1A): 325-8, 2001.
Article
em En
| MEDLINE
| ID: mdl-11299757
ABSTRACT
BACKGROUND:
To improve the radiotherapy results, we evaluated etoposide as an effective radiosensitizer by using cultured cell-lines. MATERIALS ANDMETHODS:
Four cell lines having different doubling times (DT) were used V79 (Chinese hamster fibroblasts, DT = 9 hours), (1), T24 (human bladder cancer, DT = 19 hours) (2), MDA-MB231 (human breast cancer, DT = 25-30 hours) (3) and RMG1 (human ovarian cancer, DT = 50 hours) (4). Cell survival was determined by colony assay and cell cycle analysis was performed by flow-cytometry.RESULTS:
The survival curves showed RMG1 to be the most radiosensitive, followed by MDA-MB231, T24, and V79. V79 was most chemosensitive to etoposide, followed by T24, MDA-MB231 and RMG1. Neither 24-hours exposure to etoposide (< or = 0.05 microgram/ml) or 0.5-h exposure (< or = 1.0 microgram/ml) had any cell killing effect on any of the cell lines used. When the cells were irradiated after exposure to 1 microgram/ml of etoposide for 0.5 hours, no radiosensitization was observed in any of the cell lines except V79. Enhanced radiosensitivity was observed in V79 and T24 cells (which have a relatively short DT) when they were incubated with 0.05 microgram/ml etoposide for 24 hours but no enhanced effect was seen in MDA-MB231 or RMG1 cells (which have a relatively long DT).CONCLUSION:
It is suggested that a combination of radiation and etoposide may be useful in the treatment of rapidly growing cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Radiossensibilizantes
/
Etoposídeo
/
Neoplasias
/
Antineoplásicos Fitogênicos
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2001
Tipo de documento:
Article