5-Methoxysalicylic acid and spermine: a new matrix for the matrix-assisted laser desorption/ionization mass spectrometry analysis of oligonucleotides.
J Am Soc Mass Spectrom
; 12(4): 456-62, 2001 Apr.
Article
em En
| MEDLINE
| ID: mdl-11322192
ABSTRACT
5-Methoxysalicylic acid (MSA) is demonstrated to be a useful matrix for matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrometry of oligonucleotides, when desorption/ionization without fragmentation is desired. When MSA is combined with the additive spermine, the need for desalting is reduced. The MSA/spermine matrix yields linear TOF mass spectra with improved resolution, less fragmentation, and less intense alkali ion adduct peaks than those spectra obtained using 3-hydroxypicolinic acid and 6-aza-2-thiothymine with spermine or diammonium hydrogen citrate as additives. Instrumental conditions are discussed to improve the spectral resolution, specifically the use of longer delay times in the delayed-extraction ion source.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Espermina
/
Salicilatos
/
Ácido Cítrico
/
Compostos de Amônio Quaternário
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
2001
Tipo de documento:
Article