Bioefficacy of beta-carotene dissolved in oil studied in children in Indonesia.
Am J Clin Nutr
; 73(5): 949-58, 2001 May.
Article
em En
| MEDLINE
| ID: mdl-11333850
ABSTRACT
BACKGROUND:
More information on the bioefficacy of carotenoids in foods ingested by humans is needed.OBJECTIVE:
We aimed to measure the time required for isotopic enrichment of beta-carotene and retinol in serum to reach a plateau, the extent of conversion of beta-carotene dissolved in oil with use of beta-carotene and retinol specifically labeled with 10 (13)C atoms, and the intraindividual variation in response.DESIGN:
Indonesian children aged 8--11 y (n = 35) consumed 2 capsules/d, 7 d/wk, for < or =10 wk. Each capsule contained 80 microg [12,13,14,15,20,12',13',14',15',20'-(13)C(10)]beta-carotene and 80 microg [8,9,10,11,12,13,14,15,19,20-(13)C(10)]retinyl palmitate. Three blood samples were drawn per child over a period of < or =10 wk. HPLC coupled with atmospheric pressure chemical ionization liquid chromatography-mass spectrometry was used to measure the isotopic enrichment in serum of retinol with [(13)C(5)]retinol and [(13)C(10)]retinol and of beta-carotene with [(13)C(10)]beta-carotene. The beta-carotene in the capsules used had a cis-trans ratio of 31.RESULTS:
Plateau isotopic enrichment was reached by day 21. The amount of beta-carotene in oil required to form 1 microg retinol was 2.4 microg (95% CI 2.1, 2.7). The amount of all-trans-beta-carotene required to form 1 microg retinol may be lower.CONCLUSIONS:
The efficiency of conversion of this beta-carotene in oil was 27% better than that estimated previously (1.0 microg retinol from 3.3 microg beta-carotene with an unknown cis-trans ratio). The method described can be extended to measure the bioefficacy of carotenoids in foods with high precision, requiring fewer subjects than other methods.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vitamina A
/
Beta Caroteno
Tipo de estudo:
Clinical_trials
Limite:
Child
/
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Am J Clin Nutr
Ano de publicação:
2001
Tipo de documento:
Article