In vivo antitumor effect of herpes simplex virus thymidine kinase gene therapy in rat hepatocellular carcinoma: feasibility of adenovirus-mediated intra-arterial gene delivery.
Mol Cells
; 11(2): 170-8, 2001 Apr 30.
Article
em En
| MEDLINE
| ID: mdl-11355697
ABSTRACT
Transfer of the herpes simplex virus-thymidine kinase gene, followed by the administration of ganciclovir (HSV-tk/GCV), has been a major approach for cancer gene therapy. We investigated the antitumor effect of the HSV-tk/GCV strategy with the rat orthotopic hepatocellular carcinoma (HCC) model and the tumor-selective gene delivery by an adenovirus-mediated gene transfer through the hepatic artery. The complete antitumor effect was demonstrated, after the treatment with GCV in rat HCC established by the implantation of HSV-tk transferred rat HCC cells. The in vivo bystander effect was also observed. The marked infiltration of CD4+ and CD8+ T lymphocytes, macrophages and NK cells were found in the tumor area. After the injection of adenovirus carrying the LacZ gene into the hepatic artery, the selective expression of transgene in the tumor cell was achieved. These findings indicate that the HSV-tk/GCV strategy, using an adenoviral vector, could be a promising avenue for the treatment of hepatocellular carcinoma.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timidina Quinase
/
Terapia Genética
/
Simplexvirus
/
Técnicas de Transferência de Genes
/
Neoplasias Hepáticas Experimentais
Tipo de estudo:
Prognostic_studies
Aspecto:
Implementation_research
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Mol Cells
Ano de publicação:
2001
Tipo de documento:
Article