Determination of malondialdehyde by capillary electrophoresis, application to human plasma and relation of its levels with prematurity.
Biomed Chromatogr
; 15(4): 287-91, 2001 Jun.
Article
em En
| MEDLINE
| ID: mdl-11438973
ABSTRACT
Malondialdehyde (MDA) is considered as the most important marker for monitoring lipid peroxidation, which is strongly associated with the development of serious diseases in adults and premature neonates. In this paper we report a method for determination of free MDA in human plasma using capillary zone electrophoresis. MDA was separated and determined as conjugate with tetrabutylammonium hydrogen sulphate (TBAS). Analysis was performed using 20 mM borare, pH = 9.3, as operating buffer and detection of the MDA-TBAS adduct at 267 nm. The method has a linear range up to 80 microM with a detection limit of 0.2 microM. The method was applied to the analysis of MDA in plasma of healthy adults, normal-gestation infants and of preterm neonates. Plasma proteins were successfully removed following centrifugation through a centricon-3 membrane. Results showed that the method can be easily and accurate applied for the determination of MDA in human plasma and that the level of MDA in pretern neonates is significantly higher (p
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Malondialdeído
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Humans
/
Newborn
Idioma:
En
Revista:
Biomed Chromatogr
Ano de publicação:
2001
Tipo de documento:
Article