Increased constriction of the ductus arteriosus with combined administration of indomethacin and L-NAME in fetal rats.
Biol Neonate
; 80(1): 64-7, 2001 Jul.
Article
em En
| MEDLINE
| ID: mdl-11474152
ABSTRACT
We studied age-related changes and the caliber of the ductus arteriosus (DA) after two-pathway inhibition of prostaglandin E(2) and nitric oxide (NO) by the combined administration of indomethacin, a cyclooxygenase inhibitor, and N(G)-nitro-L-arginine methyl ester (L-NAME), an NO synthase inhibitor, in fetal rats. Pregnant rats from day 18 to 21 of gestation were used. They were administered indomethacin orally (3 mg/kg) 3 h before cesarean section, and then L-NAME (50 mg/kg) was injected intraperitoneally 3 h before the rats were killed. Using rapid-freezing and shaving methods, the caliber of the DA in fetal rats was measured. Compared with the indomethacin alone group, indomethacin plus L-NAME further constricted the DA after indomethacin and L-NAME were simultaneously administered 3 h before the rats were sacrificed. The extent of the final DA constriction was almost equal to the addition of each effect of indomethacin and L-NAME. We concluded that the magnitude of DA constriction following indomethacin plus L-NAME was due to the additive effects of these agents, suggesting a possible method to treat patent DA in premature infants.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Indometacina
/
Inibidores de Ciclo-Oxigenase
/
NG-Nitroarginina Metil Éster
/
Canal Arterial
/
Inibidores Enzimáticos
Limite:
Animals
Idioma:
En
Revista:
Biol Neonate
Ano de publicação:
2001
Tipo de documento:
Article