Juvenile hormone binding protein and transferrin from Galleria mellonella share a similar structural motif.
Biol Chem
; 382(7): 1027-37, 2001 Jul.
Article
em En
| MEDLINE
| ID: mdl-11530933
ABSTRACT
It has been previously suggested that juvenile hormone binding protein(s) (JHBP) belongs to a new class of proteins. In the search for other protein(s) that may contain structural motifs similar to those found in JHBP, hemolymph from Galleria mellonella (Lepidoptera) was chromatographed over a Sephadex G-200 column and resulting fractions were subjected to SDS-PAGE, transferred onto nitrocellulose membrane and scanned with a monoclonal antibody, mAb 104, against hemolymph JHBP. Two proteins yielded a positive reaction with mAb 104, one corresponding to JHBP and the second corresponding to a transferrin, as judged from N-terminal amino acid sequencing staining. Transferrin was purified to about 80% homogeneity using a two-step procedure including Sephadex G-200 gel filtration and HPLC MonoQ column chromatography. Panning of a random peptide display library and analysis with immobilized synthetic peptides were applied for finding a common epitope present in JHBP and the transferrin molecule. The postulated epitope motif recognized by mAb 104 in the JHBP sequence is RDTKAVN, and is localized at position 82-88.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transferrina
/
Proteínas de Transporte
/
Proteínas de Insetos
/
Lepidópteros
Limite:
Animals
Idioma:
En
Revista:
Biol Chem
Ano de publicação:
2001
Tipo de documento:
Article