Analysis of VpreB expression during B lineage differentiation in lambda5-deficient mice.
J Immunol
; 167(7): 3734-9, 2001 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-11564789
ABSTRACT
The VpreB/lambda5 surrogate L chain complex is an essential component of the pre-B cell receptor, the expression of which serves as an important checkpoint in B cell development. Surrogate L chains also may serve as components of murine pro-B cell receptors whose function is unknown. We have produced two new mAbs, R3 and R5, that recognize a different VpreB epitope than the one recognized by the previously described VP245 anti-mouse VpreB Ab. These Abs were used to confirm the expression of surrogate L chains on wild-type pro-B and pre-B cell lines. Although undetectable on the cell surface, VpreB was found to be normally expressed within B lineage cells of lambda5-deficient mice. Nevertheless, VpreB expression was extinguished at the B cell stage of differentiation in these mice. The normal pattern of VpreB expression in lambda5-deficient mice excludes an essential role for pro-B and pre-B cell receptors in VpreB regulation.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas de Membrana
/
Linfócitos B
/
Cadeias lambda de Imunoglobulina
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
2001
Tipo de documento:
Article