Artificially induced unusual shape of erythrocytes: an atomic force microscopy study.
J Microsc
; 204(Pt 1): 46-52, 2001 Oct.
Article
em En
| MEDLINE
| ID: mdl-11580812
ABSTRACT
We used air operating atomic force microscopy (AFM) to study several morphological modifications of human erythrocytes, artificially produced by addition of exogenous agents including phospholipids, low ionic strength media and drugs. Most experiments were performed on unfixed samples to avoid treating red blood cells (RBCs) with chemical agents that can, in principle, induce morphological alteration. After detailed quantitative AFM characterization, the artificially produced abnormally shaped RBCs were compared with cells that occur with high incidence in blood pathologies. This morphological approach suggests a new strategy to describe and understand the biochemical and/or mechanical modifications responsible for the underlying pathologically induced changes and prove AFM to be a suitable tool to study erythrocyte deformation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Clorpromazina
/
Microscopia de Força Atômica
/
Eritrócitos
Limite:
Humans
Idioma:
En
Revista:
J Microsc
Ano de publicação:
2001
Tipo de documento:
Article