Speciation of human microsporidia by polymerase chain reaction single-strand conformation polymorphism.
Am J Trop Med Hyg
; 65(4): 397-401, 2001 Oct.
Article
em En
| MEDLINE
| ID: mdl-11693891
ABSTRACT
We describe the application of single-strand conformation polymorphism (SSCP) analysis to the speciation of human microsporidia after polymerase chain reaction (PCR) amplification with the panmicrosporidian primers PMP1 and PMP2. We compared the DNA extracted and amplified from different genotypes or isolates of Enterocytozoon bieneusi, Encephalitozoon cuniculi, E. hellem, and E. intestinalis plus an isolate of Vittaforma corneae. The PCR-SSCP, when performed at 20 degrees C, generated 2 bands in distinctive, reproducible patterns in polyacrylamide gels for each species of microsporidia tested, regardless of genotype or isolate. We found PCR-SSCP to be an easy and reproducible method for speciation of human microsporidia when the primer pair PMP1 and PMP2 is used.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
DNA de Protozoário
/
Microsporidiose
/
Microsporídios
/
Proteínas de Membrana
/
Proteínas do Tecido Nervoso
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2001
Tipo de documento:
Article