Diagnostic methods for detecting forms and strains of Helicobacter pylori and evaluation of its eradication.
Bull Exp Biol Med
; 132(2): 802-6, 2001 Aug.
Article
em En
| MEDLINE
| ID: mdl-11713571
ABSTRACT
Diagnostic methods for detecting forms and strains of Helicobacter pylori isolated from biopsy specimens of gastric mucosa in 28 patients with duodenal ulcers and evaluation of its eradication were compared. Biopsy specimens from all patients were tested for the presence of H. pylori by the urease test, histological method, and PCR with species-specific primers before and after treatment. H. pylori infection was detected in all patients before treatment, the mean titer of serum IgG being 36.7+/-16.6 U/ml. Biopsy specimens positive for H. pylori in PCR were subjected to restriction analysis of specific PCR-amplified genes or their fragments. The fingerprint analysis gave electrophoregrams of restriction products amplified fragment of flaA gene of H. pylori in 7 patients. Differences in restriction maps indicate the presence of 5 H. pylori strains in the studied samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ranitidina
/
Helicobacter pylori
/
Infecções por Helicobacter
/
Úlcera Duodenal
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Bull Exp Biol Med
Ano de publicação:
2001
Tipo de documento:
Article