The Cdk5-p35 kinase associates with the Golgi apparatus and regulates membrane traffic.
EMBO Rep
; 2(12): 1139-44, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11743029
ABSTRACT
We show here that an active Cdk5-p35 kinase is present in Golgi membranes, where it associates with a detergent-insoluble fraction containing actin. In addition, Cdk5-p35-dependent phosphorylation of alpha-PAK immunoreactive protein species was detected in Golgi membranes, as well as an interaction with the small GTPase, Cdc42. Moreover, antisense oligonucleotide suppression of Cdk5 or p35 in young cultured neurons, as well as inhibition of Cdk5 activity with olomoucine, blocks the formation of membrane vesicles from the Golgi apparatus. Taken together, these results show a novel subcellular localization of this kinase and suggest a role for Cdk5-p35 in membrane traffic during neuronal process outgrowth.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Celular
/
Quinases Ciclina-Dependentes
/
Complexo de Golgi
/
Membranas Intracelulares
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
EMBO Rep
Ano de publicação:
2001
Tipo de documento:
Article