Improvement in the apparent mass resolution of oligonucleotides by using 12C/14N-enriched samples.
Anal Chem
; 74(1): 226-31, 2002 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-11795798
ABSTRACT
The apparent mass resolution of oligonucleotides in time-of-flight (TOF) mass spectrometers has been examined. In a reflectron TOF instrument, where the isotopic profile can be completely resolved, the apparent resolution matches the instrument's resolving power. In a linear TOF instrument, unresolved isotopic profiles limit the apparent resolution to much lower values than the actual instrument resolution. By using 12C/14N-enriched oligonucleotides, the apparent resolution can be improved significantly. The isotope enrichment method also enhances the signal-to-noise ratio.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2002
Tipo de documento:
Article