Rapid and sensitive method for the analysis of carbon monoxide in blood using gas chromatography with flame ionisation detection.
J Chromatogr B Analyt Technol Biomed Life Sci
; 766(1): 115-21, 2002 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-11820286
ABSTRACT
In order to measure changes in physiological CO concentrations in blood with good accuracy, a method was developed using gas chromatography with flame ionisation detection (250 degrees C). A nickel catalyst system was fitted to convert CO to methane at 375 degrees C after separation with a molecular sieve column at 35 degrees C. Helium was used as carrier at 30 ml/min. Porcine or human blood (400 microl) was sampled in gastight tubes and treated with sulfuric acid and saponin (800 microl). Accuracy was 1.4% and 1.5% (RSD), respectively. Precision was 2.8% (porcine blood). Limit of detection was 0.01 nmol/ml gas and limit of quantification 12 nmol/ml blood. Calibration was made in the interval 12-514 nmol/ml blood (corresponding to 0.1-6% COHb). Samples were stable for at least a month at +4 degrees C. This paper describes a method with high sensitivity and good accuracy, suitable for analysis of low CO concentrations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monóxido de Carbono
/
Cromatografia Gasosa
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Ano de publicação:
2002
Tipo de documento:
Article