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Cross-species microsatellite markers for elucidating population genetic structure in Arabidopsis and Arabis (Brassicaeae).
Clauss, M J; Cobban, H; Mitchell-Olds, T.
Afiliação
  • Clauss MJ; Department of Genetics and Evolution, Max Planck Institute of Chemical Ecology, Beutenberg Campus, Winzerlaer Str. 10, 07745 Jena, Germany. clauss@ice.mpg.de
Mol Ecol ; 11(3): 591-601, 2002 Mar.
Article em En | MEDLINE | ID: mdl-11918792
ABSTRACT
Species closely related to model organisms present the opportunity to efficiently apply molecular and functional tools developed by a large research community to taxa with different ecological and evolutionary histories. We complied 42 microsatellite loci that amplify under common conditions in four closely related Arabidopsis A. thaliana; A. halleri; A. lyrata ssp. lyrata; and A. lyrata ssp. petraea, as well as in one more distantly related crucifer; Arabis drummondii. Variation at these loci is amenable to a diversity of applications including population genetics, phylogeographical analyses, mapping of inter and intraspecific crosses, and recombination mapping. Our analysis of microsatellite variation illustrates significant differences in population genetic parameters among three Arabidopsis species. A population of A. thaliana, an inbreeding annual plant associated with disturbed habitats, was highly monomorphic (P = 8% percent polymorphic loci) and only 0.2% heterozygous for 648 locus-by-individual combinations. A population of the self-incompatible perennial herb, A. halleri, was more genetically variable (P = 71%) and had an excess of heterozygosity that may reflect a recent population bottleneck associated with human-mediated founder events. A population of the self-incompatible perennial herb, A. lyrata ssp. petraea, was even more genetically variable (P = 86%) and appeared to be at mutation-drift equilibrium. Population structure estimated from neutrally evolving loci provides an empirical expectation against which hypotheses of adaptive evolution at functional loci can be tested.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Repetições de Microssatélites / Brassicaceae Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Mol Ecol Ano de publicação: 2002 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Repetições de Microssatélites / Brassicaceae Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Mol Ecol Ano de publicação: 2002 Tipo de documento: Article