Safety and bioactivity of intracoronary delivery of naked plasmid DNA encoding human atrial natriuretic factor.
Acta Pharmacol Sin
; 23(7): 609-11, 2002 Jul.
Article
em En
| MEDLINE
| ID: mdl-12100753
ABSTRACT
AIM:
To evaluate the safety and bioactivity of catheter-mediated intracoronary gene delivery of naked plasmid DNA encoding human atrial natriuretic factor (hANF).METHODS:
hANF gene delivery was performed in 12 canines. For each canine, 4 mg of reconstructed naked plasmid DNA encoding hANF (pCR3*hANF, n=6) or pCR3 (n=6, control) in 2 mL normal saline was injected into left coronary artery via a coronary angiographic catheter. The expression of hANF mRNA in left ventricular myocardium, liver, lung, and kidney was identified by reverse transcription polymerase chain reaction and Southern blot analysis 40 d after gene delivery. Plasma ANF levels were determined by radioimmunoassay.RESULTS:
The naked pCR3*hANF caused significant expression of hANF mRNA in ventricular myocardium (P <0.01). No hANF mRNA was detected in distal tissues, including liver, lung, and kidney (P >0.05). ANF levels were significantly higher in pCR3*hANF group than those in control group.CONCLUSION:
These facts firstly demonstrate that intracoronary delivery of naked pCR3*hANF is probably a safe and feasible method for gene delivery by which hANF gene can be expressed in ventricular myocardium effectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Fator Natriurético Atrial
/
Miocárdio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta Pharmacol Sin
Ano de publicação:
2002
Tipo de documento:
Article