Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1.
Plant Physiol
; 130(1): 234-43, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12226503
ABSTRACT
To further our understanding of how plants defend against the harmful effects of ultraviolet (UV) light, we characterized an Arabidopsis mutant hypersensitive to UV-B. This mutant, UV resistance locus 8-1 (uvr8-1), contains a single recessive mutation at the bottom of chromosome 5. Fine-scale mapping localized uvr8-1 to a 21-kb locus containing five predicted open reading frames. Sequencing of this entire region revealed that the uvr8-1 allele contains a 15-nucleotide deletion in a gene similar to the human guanine nucleotide exchange factor regulator of chromatin condensation 1. This mutation reduces the UV-B-mediated induction of flavonoids and blocks chalcone synthase mRNA and protein induction. In contrast, uvr8-1 has enhanced induction of PR1 and PR5 proteins in response to UV-B, an indication of increased UV-B injury. These results suggest that UVR8 acts in a UV-B signal transduction pathway leading to induction of flavonoid biosynthesis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Transdução de Sinais
/
Arabidopsis
/
Proteínas de Ciclo Celular
/
Fatores de Troca do Nucleotídeo Guanina
/
Proteínas de Arabidopsis
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Plant Physiol
Ano de publicação:
2002
Tipo de documento:
Article