Rapid time-resolved immunofluorometric assay for the measurement of creatine kinase in serum and whole blood samples.
J Agric Food Chem
; 50(23): 6659-62, 2002 Nov 06.
Article
em En
| MEDLINE
| ID: mdl-12405757
ABSTRACT
A rapid and simple immunochemical method was developed for the assessment of the creatine kinase (MM) isoenzyme [CK(MM)], a protein marker linked with animal welfare and meat quality. The one-step time-resolved immunofluorometric assay produced quantitative results from serum or whole blood samples in 20 min. The analytical limit of detection (mean + 2s) for the immunoassay was 17 ng/mL (n = 6), and the functional limit of detection for the analysis of porcine whole blood samples was 426 ng/mL (n = 24). The working range of the method was linear up to 50 micro g/mL, and the within-assay precision varied between 2.1 and 10.9%. The analysis of porcine serum samples showed that the results from the immunoassay method and colorimetric CK enzyme activity determination were highly correlated (r(2) = 0.965, n = 17, p < 0.001). The practicability of the assay was demonstrated by the analysis of 300 porcine whole blood samples in a slaughterhouse environment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fluorimunoensaio
/
Creatina Quinase
/
Isoenzimas
Limite:
Animals
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2002
Tipo de documento:
Article