Method for inactivating and fixing unstained smear preparations of mycobacterium tuberculosis for improved laboratory safety.
J Clin Microbiol
; 40(11): 4077-80, 2002 Nov.
Article
em En
| MEDLINE
| ID: mdl-12409378
ABSTRACT
The inactivation of smears that contain Mycobacterium tuberculosis for microscopy before removal of the material from a biosafety cabinet is an important safety factor in preventing the potential transmission of tuberculosis to laboratory workers. The fixing and inactivating properties of heat flaming, 70% ethanol, and 1, 3, and 5% phenol in ethanol for smears containing M. tuberculosis were investigated. Heat flaming failed to inactivate the smear material, whereas 5% phenol in ethanol successfully fixed and inactivated all smears containing M. tuberculosis both from concentrated sputum samples and from culture material.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Segurança
/
Manejo de Espécimes
/
Fixadores
/
Temperatura Alta
/
Laboratórios
/
Mycobacterium tuberculosis
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2002
Tipo de documento:
Article