Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.
Science
; 298(5600): 2006-10, 2002 Dec 06.
Article
em En
| MEDLINE
| ID: mdl-12471263
ABSTRACT
The Frizzled-2 receptor (Rfz2) from rat binds Wnt proteins and can signal by activating calcium release from intracellular stores. We show that wild-type Rfz2 and a chimeric receptor consisting of the extracellular and transmembrane portions of the beta2-adrenergic receptor with cytoplasmic domains of Rfz2 also signaled through modulation of cyclic guanosine 3',5'-monophosphate (cGMP). Activation of either receptor led to a decline in the intracellular concentration of cGMP, a process that was inhibited in cells treated with pertussis toxin, reduced by suppression of the expression of the heterotrimeric GTP-binding protein (G protein) transducin, and suppressed through inhibition of cGMP-specific phosphodiesterase (PDE) activity. Moreover, PDE inhibitors blocked Rfz2-induced calcium transients in zebrafish embryos. Thus, Frizzled-2 appears to couple to PDEs and calcium transients through G proteins.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Receptores de Neurotransmissores
/
GMP Cíclico
/
Diester Fosfórico Hidrolases
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
2002
Tipo de documento:
Article