Expression of functional prostaglandin D (DP) receptors in human corpus cavernosum smooth muscle.
Int J Impot Res
; 14(6): 446-52, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12494276
ABSTRACT
Prostaglandin D(2) (PGD(2)) binds to specific G-protein coupled receptors (DP) and induces smooth muscle relaxation by stimulating the synthesis of intracellular cAMP. In this study, we examined the role of PGD(2) and DP receptors in regulating human penile smooth muscle contractility. We determined that human corpus cavernosum tissue and smooth muscle cells in culture expressed functional DP receptor and lipocalin-like prostaglandin D synthase by reverse-transcribed polymerase chain reaction (RT-PCR). Functional PGD synthase activity was confirmed by the synthesis of PGD(2) in human corpus cavernosum smooth muscle cells upon addition of exogenous arachidonic acid. Organ bath preparations of human corpus cavernosum tissue strips, contracted with phenylephrine, relaxed in a dose-dependent fashion to either PGD(2) or the DP selective agonist BW245C. Cultures of human corpus cavernosum smooth muscle cells treated with BW245C showed a two-fold increase in cAMP synthesis. These data are consistent with the expression of functional DP receptors in human corpus cavernosum. This suggests the presence of an intact prostanoid autocrine system that may play a role in regulating penile erectile function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pênis
/
Receptores de Prostaglandina
/
Receptores Imunológicos
/
Músculo Liso Vascular
Limite:
Humans
/
Male
Idioma:
En
Revista:
Int J Impot Res
Ano de publicação:
2002
Tipo de documento:
Article